In tissue engineering applications, the cross talk between cells and their environment is critical for successful cell adhesion and proliferation during cell culture. Flat surfaces of traditional 2D plates and culture flasks, while suitable, do not fully mimic the physical and geometrical intricacies of their in vivo environment. With advances in tissue engineering and the requirement to develop more physiologically relevant conditions for cell culture, 3D culture scaffolds have emerged that enable the growth of cells in 3D architectures (Greiner et al., Current Tissue Engineering, 2013). This is important for applications such as organ engineering where understanding and controlling the physical characteristics of the scaffold is critical (Evangelatov and Pankov, Regenerative Medicine and Tissue Engineering, 2013).
The benefits of 3D over 2D culture are numerous: gone is the inert rigidness of 2D substrates in favor of an envrionment that mimics native tissues, moreover, growth factor diffusion is slow in 3D cultures which promotes cell-cell communication. Akron recognizes the importance of the third dimension in cell culture and has, as a result, developed Ak-PolyFibers – patent-pending electrospun fiber multiwell plates for cell culture and cancer research. The system is based on polymer nanofibers integrated into standard multi-well plates or cell culture dishes which enable, among others, accurate observation of effects of various chemical compounds on cell behavior as well more robust and controlled cell differentiation and expansion.
All that’s left is to try them yourself and appreciate the difference. To find out more about Polyfibers and associated proteins for cell growth and proliferation in a 3D environment contact us at firstname.lastname@example.org.